基金项目：高等学校博士学科点专项科研基金(20101106110031) 作者简介：全毅(1985-)，男，硕士，主要研究方向：脑肿瘤治疗 通信联系人：高钟镐(1963-)，男，研究员，主要研究方向：肿瘤靶向制剂和纳米给药系统.
(1. 中国医学科学院北京协和医学院药物研究所，北京，100050;2. 延边大学药学院药剂学教研室，吉林延吉，133000;3. 中国医学科学院北京协和医学院医药生物技术研究所，北京，100050)
Construction of C6/Wistar brain glioma animal model based on in vivo bioluminescence imaging
QUAN Yi1, HUANG Wei1, SHAO Shuai2, JI Mingji1, SHAO Rongguang3, GAO Zhonggao1
(1. Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical college, Beijing, 100050, China; 2. Department of Pharmaceutics, Yanbian University, Yanji 133000, China; 3. Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union 30 Medical college, Beijing, 100050, China)
Abstract: This study aimed at constructing the C6/Wistar brain glioma animal model based on in vivo bioluminescence imaging. A stable rat glioma cell line C6-Luc expressing firefly luciferase was firstly constructed. The DAPI fluorescence labeled C6-Luc cells were then inoculated in the right caudate nucleus of Wistar rats through stereotactic surgery. After inoculation for 0d, 7d, 14d and 21d, the tumor growth was detected by a Xenogen in vivo animal imaging system. The next day record of weight and neurological score were performed. The rats brain were removed to be subjected to the analysis of conventional HE staining, GAFP, S-100 immunohistochemical staining. At the same time, C6-Luc cells were inoculated in nude mice to detect the growth of glioma. Tumor growth was detected 7 d after inoculation. Body weight and behavior of rats both had changes. The results of pathological examination showed that tumor cells can be seen along the nerve fibers to the surrounding invasion, and multiple hemorrhages were found in tumor tissues. Immunohistochemical staining showed that the expressions of glial fibrillary acidic protein, GFAP, and nerve fibers strongly positive S-100 protein and tumor cell aggregation were found, and blood-brain barrier was severely damaged along with the invasive growth of the tumor tissue to the opposite side. Most rats died within 30d. The C6/Wistar brain glioma animal model based on in vivo bioluminescence imaging was successfully constructed stably. It can be applied to evaluate anticancer drugs or gene therapy for pre-clinical study.
Key words: glioma ; animal model ; bioluminescence imaging ; luciferase;Wistar rat